The Overlooked Aspect of Biological Product Quality Control!

DNase & RNase Detection Background

With the application of mRNA synthesis, the quality control requirements in the research and process are increasing, but why the impurity residue index has been tested all over, and the product quality is still not up to standard?

It could be the nuclease!

In mRNA research and processing, DNase/RNase is used to remove host DNA/RNA residue, which is likely to cause DNase/RNase residue. Residual DNase/RNase causes substandard final product quality, lead to high-risk intensity immunogenicity and other reactions. Therefore, accurate analysis and detection of DNase/RNase residues has become one of the key points in the quality control of biological products.

Regulation of Nuclease Residue Control

Different regulations contain key points of nuclease residues control.

Progress of DNase & RNase Detection

Compared with nucleic acid hydrolytic gel electrophoresis and ultraviolet spectrophotometer, fluorescence probe method is one of the best choices for detecting DNase/RNase residual for its high sensitivity, fast detection and realizing the quantitative detection of nuclease activity.

Principle and Application of Fluorescence Probe for DNase & RNase Detection

The detection principle of DNase & RNase detection based on nucleic acid fluorescent substrate method is basically the same: DNA probe and RNA probe labeled with fluorophores are designed and mixed with test samples.

The recognition of nucleic acid fluorescence probe DNase & RNase detection is increasing.

A Quality Control Tool Besides You

Hzymes has designed and developed DNase detection kit and RNase detection kit based on nucleic acid fluorescence substrate method.

After special sequence design, the probe can identify a variety of DNase & RNase respectively, and meet the detection needs of a variety of samples in a variety of scenarios. Compared with an imported brand (fluorescent probe method), the minimum detection limits of DNase and RNase detection kits were as low as 1/8.

At the same time, the kit has gone through complete methodological verification, and the quality control is guaranteed. To test buffers or materials commonly used in molecular experiments, Hzymes Kit interferes with fewer species. For some samples with interfering substances, it can be diluted with water and tested according to the actual use scenario.

Product Features

1. Meet the detection needs of various scenarios and samples: The probe sequence is specially designed to identify a variety of DNase & RNase, which is suitable for the detection of biological products, non-nuclease consumables, non-enzyme reagents and other samples;

2.  Higher sensitivity, strong anti-interference performance;

3.  The kit has undergone complete methodological verification;

4.  Stable quality, long-term supply: batch CV<10%, batch CV<15%.