Service content
Composite Type | Gene length | Cycle (working days) | Service Description |
Conventional gene synthesis | <1.5k | 5-7 | Standard service, cloned by default in PUC57 series carriers |
1.5-3k | 8-15 | ||
3-5k | 15-20 | ||
>5K | Ask about |
Delivery results
Physical object | Electronic report |
1 tube of 2-5 μ g liquid plasmid (if the customer requests delivery of freeze-dried powder, it can be freeze-dried for free, with an additional 1-2 days of cycle) | COA file (enzyme digestion verification size correct) |
Sequencing map | |
1 tube of bacterial solution | Plasmid construction map |
Sequence alignment file (ensuring 100% accuracy of synthesized sequences) |
♦ Flexible quality control requirements: multiple default inspection items and various optional inspection items.
♦ Through the screening of receptive/monoclonal/plasmid skeletons and optimization of fermentation processes, mRNA template plasmids with high proportion of super helices, good PolyA tail stability, and high integrity can be provided.
As an indispensable tool in the field of molecular biology, plasmids play a crucial role in the success or failure of experiments. Hanhai New Enzyme can provide plasmid preparation services ranging from scientific research to industrial level, suitable for various customers from scientific research and early research to industrial production, meeting different application scenarios.
Plasmid type | Research grade | Industrial grade |
Cycle | Starting from 3 working days | Starting from 2 weeks |
A260/A280 | 1.8-2.0 | 1.8-2.0 |
Electrophoresis | √ | √ |
Enzyme digestion validation | √ | √ |
Hyper spiral ratio | × | Conventional plasmid ≥ 90% MRNA plasmid determined based on Poly (A) length |
Endotoxin | × | ＜10EU/mg |
Poly (A) guarantees | × | mRNA plasmid ensures Poly (A) |
Sequence | Optional | Optional |
IVT verification | Optional | Optional |
Process steps | Recovery (%) | Nano Conc.(ng/uL) | scDNA (%) | Endoxin (EU/mg pDNA) | HCP (ug/mg pDNA） | HCD (ug/mg pDNA） |
After clarification and filtering | / | / | / | >10 | 0.034 | / |
After AEX elution | / | 243.481 | 85 | >10 | 0.0032 | 2.4 |
After HIC elution | 75 | 63.017 | 90.5 | <10 | ND | 0.02 |
Process steps | Recovery (%) | Nano Conc.(ng/uL) | scDNA (%) | Endoxin (EU/mg pDNA) | HCP (ug/mg pDNA） | HCD (ug/mg pDNA） |
After deep filtration | / | / | / | >10 | 0.034 | / |
Concentrate and replace the liquid | / | 1058.3 | 70.5 | / | 0.00075 | 2.85 |
After AC elution | 79.2 | 173.726 | 84.6 | / | 0.0022 | 2.81 |
After AEX elution | 86.7 | 368.488 | 93.4 | / | ND | 1.63 |
pUC57-Amp | pAC19 | pCDNA3.1 Hygro(+) | pEGFP-C3 | pET-20b(+) | pET-29a(+) | pET-52b(+) | PGL3-BASIC |
pUC57-Kan | pAC19-ccdb | pCDNA3.1 myc-His A | pET-3a | pET-21a(+) | pET-29b(+) | pETDuet-1 | pGL3-control |
pUC57-Kan-ccdB | pAC19-XB | pCDNA3.1(-) | pET-3d | pET-21b(+) | pET-30a(+) | PETM-11 | PGL4.10 |
PUC57simple | pATX1 | pcDNA3.1(+) | pET-9a | pET-22b(+) | pET-32a(+) | PET-SUMO | PHT43 |
pACYCDuet-1 | pATX-SUMO | PCDNA3.1(+)-EGFP | pET-1la | pET-24a(+) | pET-41a(+) | pFastBac Dual | PJET-1.2 |
pCDFDuet-1 | pBF | pCDNA3.1(+)C-HA | pET-11d | pET-24b(+) | pET-43.1a(+) | Pfastbacl | PLVX-PURO |
pEGFP-N1 | pBluescriptIl SK(+) | PCOLDII | pET-15b | pET-25b(+) | pET-44a(+) | PGADT7 | PMAL-C2X |
pQE-30 | pCAMBIA1301 | pDONR207 | pET-16b | pET-26b(+) | pET-44b(+) | PGBKT7 | PMAL-C5X |
PQE-60 | PCCI-2U | pDONR223 | pET-17b | pET-27b(+) | pET-45b(+) | PGEX-4T-1 | PMAL-P5E |
pTT5 | PCDH-CMV-EF1A-EGFP-T2A-PURO | pEGFP-C1 | PET-19b(+) | pET-28a(+) | pET-48b(+) | PGEX-6P-1 | PML104 |
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