丰富的蛋白表达平台和技术储备，完备的发酵、纯化、蛋白评测平台，稳定的交付保障

Insect Expression System Service

Insect Expression System Service

Insect expression system is a eukaryotic system with a wide range of applications, which has the ability for post-translational modifications and expression of foreign proteins similar to most eukaryotic expression systems. It is suitable for expression of proteins that are toxic to mammalian cell.

Compared with mammalian cell system, Insect expression system has similar translation and post-translational modifications patterns and abilities. The advantages for insect expression system are simple cultivation requirements and available for suspension culture as well as easy to achieve massive production.

· Workflow ·

Mature protein expression Platform and technique

· Having a rational design and directed evolutionary molecular design platform, efficiently and accurately implementing gene sequence design and optimization
· Suitable for expressing some proteins that may have negative effects on host cells of dairy animals, such as kinases and toxic proteins.

Perfect fermentation, purification, protein evaluation platform

· The Bac to Bac system for protein expression, protein stewing platform technology, and rich experience in cell culture and purification process optimization. It combines rapid process development and in-depth optimization development.
·A complete egg self quality control system can comprehensively evaluate protein performance

Guarantee to Delivery

· Rich protein expression experience: self-developed+CRO
· Professional team+platform operation: doctoral/master's/undergraduate team improvement: molecular design platform/fermentation platform/purification platform/protein evaluation platform operation
· ·Complete experimental equipment and partitioned experimental environment: A fully functional partitioned experimental site with over 300 research and development equipment to support diverse customer needs

- DELIVERY STANDARDS -

Service content	Delivery content	Period
Sequence optimization and vector construction	Sequence information and expression plasmids	2-3Weeks
Virus amplification	P2 virus (5-10ml)	2-3Weeks
MOI optimization	Optimization report	2-3Weeks
Small scale shaking bottle expression	Protein sample and expression evaluation report	2-3Weeks

- CASE -

Case Description:The case customer needed to express a cytokine, and tried to express this factor in many expression systems, all of which showed low biological activity and low expression and not be detected. Hzymes According to the analysis of protein characteristics, it is recommended to choose the insect baculovirus expression system for expression, and the expression amount can reach 100 mg/L. The final obtained protein has normal biological activity to meet the needs of customer.

Case 1: Advantageous platform technology facilitates efficient expression of cytokines