Hzyme’s Recombinant Protein Expression CRDMO Services
Protein sequence design & Strain construction
· Specializes in Escherichia coli, Pichia yeast Strain construction technology
· Strain building library PCB + MCB + WCB
· Protein sequence survey and design,
· Optimization of the soluble expression
· Development and optimization of the fermentation process
· Purification process development and optimization
· Preparation process development and optimization
Development & Optimization of downstream processing
Large-scale production and manufacturing
· Fermentation amplification and production 5L-50L-500L-2.5t-25t Fermenter
· Purification, amplification and productionD75-D100-D200-D300-D450-D800 chromatography column
· Freeze production0.2 ㎡ -0.5 ㎡ -2 ㎡ -40 ㎡ lyophilizer

Strain development and library building services
Process development and service optimization
Flexible production capacity matching
Protein multidimensional quality control platform
Perfect quality control platform
· quality specification study
· Method development and validation
· Sample commissioned testing
· Stability research
2-4 Weeks
Small scale process validation
· Process repetition
· Assof process magnificability
· Existing product quality status quo
As short as 1-2 months
Fermentation, purification process development
Quality inspection method development and transfer
· Establishment of the seed bank (optional)
· Fermentation process optimization and amplification
· The purification process is optimized and amplified
· Transfer / development of quality inspection methods (optional)
1-2 Months
Amplification process verification GMP volume production
· Quality control method for transfer to the quality department
· The fermentation and purification process was transferred to the production sector
· 3 batches of amplification process validation
· GMP Manufacturing for delivery
Short to 3 months for efficient delivery of GMP level products (analytical method development and transfer cycle may affect the overall project development cycle)
- CASE -
Case Description:A pharmaceutical company customer needs prepare a large numbers of low cost of preparation g-grade proteins at low cost, requiring purity >95%, endotoxin and other residues to reach the level of pharmaceutical proteins. Hzymes developed a 3-step chromatography scheme using the platform dominant E. coli expression system,with expression levels of 20 mg/g wet bacteria, purity > 98%, endotoxin content < 0.1 EU/mg, and single-batch production capacity of hundreds of grams. At present, tens of millions of orders have been stably delivered.
Case 1: Scale manufacturing of high-purity and low-endotoxin medicinal proteins
Case Description:A famous domestic molecular diagnosis company needs a special molecular enzyme raw materials, enzyme raw material sequence was known. However, the customer stated that there were difficulties in developing temperature control processes, scaling up processes, poor product stability, and limited mass production capacity during their own research and sample production processes, which directly affect the development of downstream testing reagents and supporting instruments. Therefore, Hzymes Biotech was entrusted to participate in the cooperative research, improve the process and implement the mass production of this raw material. After process optimization, the purity has been increased to more than 95%, about 1.8 times higher than the vitality. The consistency between batches is high, and the production capacity of a single batch has been increased by more than 4 times, which has been highly recognized by the customer.
Case 2: Cooperation of high purity and low residual molecular enzymes
MRNA vaccines and drug-related services
Recombinant Protein
Expression CRDMO Services
Biopharmaceutical raw materials and quality control reagent kits
Service subscription
Website & Brochure
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